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Premier Biosoft primer premier 5.0
Primer Premier 5.0, supplied by Premier Biosoft, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+5%2E0/primer+premier+5+0/pm40665687-61-14-17
Average 90 stars, based on 1 article reviews
primer premier 5.0 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae).
Article Snippet: Primer 5.0 (http://www.premierbiosoft.com/, accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Molecular mechanisms based on peripheral level of vanillin recognition in Orthaga achatina (Lepidoptera: Pyralidae).
Article Snippet: Orthaga achatina (Lepidoptera Pyralidae) is a specialist pest of the camphor tree Cinnamomum camphora.. Vanillin is a volatile compound found in many plants, and its effects on insects can be either attractive or repellent, depending on the species.. However, the behavioral response of Orthaga achatina to vanillin, a volatile compound emitted by camphor trees, remains unexplored.

Article Title: Mechanism of circRNA_4083 Circularization and Its Role in Regulating Cell Viability.
Article Snippet: Primers were designed using Primer 5.0 (PREMIER Biosoft, San Francisco, CA, USA) and Oligo 7 (Molecular Biology Insights, San Francisco, CA, USA) with GAPDH as the endogenous control.

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae)
Article Snippet: Primer 5.0 ( http://www.premierbiosoft.com/ , accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Decoding the Olfactory Deception: Molecular Basis into Chilo suppressalis ' Recognition of the Lethal Trap Plant Vetiveria zizanioides .
Article Snippet: The striped stem borer (Chilo suppressalis), a devastating rice (Oryza sativa L.) pest, exhibits a paradoxical oviposition preference for vetiver grass (Vetiveria zizanioides L.), a nonhost plant causing complete larval mortality.. To elucidate the molecular basis of this maladaptive attraction, we investigated the olfactory mechanisms driving C. suppressalis’ recognition of vetiver volatiles.. Experimental results demonstrated that C. suppressalis exhibited a significant oviposition preference for vetiver over its natural host rice, despite the developmental incompatibility of offspring on the nonhost plant.

Article Title: Key Volatiles and ORs Mediating Oviposition Preference for Maize Plants in Chinese Population of Spodoptera frugiperda .
Article Snippet: Spodoptera frugiperda is a global pest that shows distinct preferences between maize and rice hosts.. Concerning the prevailing S. frugiperda corn strains in China, we identified five maize-specific volatiles through GC-MS analysis of volatiles from maize and rice seedlings, in which benzothiazole and nonadecane were confirmed as key volatiles mediating attractants for female moths.. Then, we screened for candidate odorant receptors (ORs) by analyzing differentially expressed genes between the moths exposed and unexposed to the active volatiles.

Cloning:

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae).
Article Snippet: Primer 5.0 (http://www.premierbiosoft.com/, accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Molecular mechanisms based on peripheral level of vanillin recognition in Orthaga achatina (Lepidoptera: Pyralidae).
Article Snippet: Orthaga achatina (Lepidoptera Pyralidae) is a specialist pest of the camphor tree Cinnamomum camphora.. Vanillin is a volatile compound found in many plants, and its effects on insects can be either attractive or repellent, depending on the species.. However, the behavioral response of Orthaga achatina to vanillin, a volatile compound emitted by camphor trees, remains unexplored.

Article Title: Mechanism of circRNA_4083 Circularization and Its Role in Regulating Cell Viability.
Article Snippet: Primers were designed using Primer 5.0 (PREMIER Biosoft, San Francisco, CA, USA) and Oligo 7 (Molecular Biology Insights, San Francisco, CA, USA) with GAPDH as the endogenous control.

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae)
Article Snippet: Primer 5.0 ( http://www.premierbiosoft.com/ , accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Decoding the Olfactory Deception: Molecular Basis into Chilo suppressalis ' Recognition of the Lethal Trap Plant Vetiveria zizanioides .
Article Snippet: The striped stem borer (Chilo suppressalis), a devastating rice (Oryza sativa L.) pest, exhibits a paradoxical oviposition preference for vetiver grass (Vetiveria zizanioides L.), a nonhost plant causing complete larval mortality.. To elucidate the molecular basis of this maladaptive attraction, we investigated the olfactory mechanisms driving C. suppressalis’ recognition of vetiver volatiles.. Experimental results demonstrated that C. suppressalis exhibited a significant oviposition preference for vetiver over its natural host rice, despite the developmental incompatibility of offspring on the nonhost plant.

Article Title: Key Volatiles and ORs Mediating Oviposition Preference for Maize Plants in Chinese Population of Spodoptera frugiperda .
Article Snippet: Spodoptera frugiperda is a global pest that shows distinct preferences between maize and rice hosts.. Concerning the prevailing S. frugiperda corn strains in China, we identified five maize-specific volatiles through GC-MS analysis of volatiles from maize and rice seedlings, in which benzothiazole and nonadecane were confirmed as key volatiles mediating attractants for female moths.. Then, we screened for candidate odorant receptors (ORs) by analyzing differentially expressed genes between the moths exposed and unexposed to the active volatiles.

Expressing:

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae).
Article Snippet: Primer 5.0 (http://www.premierbiosoft.com/, accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Molecular mechanisms based on peripheral level of vanillin recognition in Orthaga achatina (Lepidoptera: Pyralidae).
Article Snippet: Orthaga achatina (Lepidoptera Pyralidae) is a specialist pest of the camphor tree Cinnamomum camphora.. Vanillin is a volatile compound found in many plants, and its effects on insects can be either attractive or repellent, depending on the species.. However, the behavioral response of Orthaga achatina to vanillin, a volatile compound emitted by camphor trees, remains unexplored.

Article Title: Mechanism of circRNA_4083 Circularization and Its Role in Regulating Cell Viability.
Article Snippet: Primers were designed using Primer 5.0 (PREMIER Biosoft, San Francisco, CA, USA) and Oligo 7 (Molecular Biology Insights, San Francisco, CA, USA) with GAPDH as the endogenous control.

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae)
Article Snippet: Primer 5.0 ( http://www.premierbiosoft.com/ , accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Decoding the Olfactory Deception: Molecular Basis into Chilo suppressalis ' Recognition of the Lethal Trap Plant Vetiveria zizanioides .
Article Snippet: The striped stem borer (Chilo suppressalis), a devastating rice (Oryza sativa L.) pest, exhibits a paradoxical oviposition preference for vetiver grass (Vetiveria zizanioides L.), a nonhost plant causing complete larval mortality.. To elucidate the molecular basis of this maladaptive attraction, we investigated the olfactory mechanisms driving C. suppressalis’ recognition of vetiver volatiles.. Experimental results demonstrated that C. suppressalis exhibited a significant oviposition preference for vetiver over its natural host rice, despite the developmental incompatibility of offspring on the nonhost plant.

Article Title: Key Volatiles and ORs Mediating Oviposition Preference for Maize Plants in Chinese Population of Spodoptera frugiperda .
Article Snippet: Spodoptera frugiperda is a global pest that shows distinct preferences between maize and rice hosts.. Concerning the prevailing S. frugiperda corn strains in China, we identified five maize-specific volatiles through GC-MS analysis of volatiles from maize and rice seedlings, in which benzothiazole and nonadecane were confirmed as key volatiles mediating attractants for female moths.. Then, we screened for candidate odorant receptors (ORs) by analyzing differentially expressed genes between the moths exposed and unexposed to the active volatiles.

Clone Assay:

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae).
Article Snippet: Primer 5.0 (http://www.premierbiosoft.com/, accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Molecular mechanisms based on peripheral level of vanillin recognition in Orthaga achatina (Lepidoptera: Pyralidae).
Article Snippet: Orthaga achatina (Lepidoptera Pyralidae) is a specialist pest of the camphor tree Cinnamomum camphora.. Vanillin is a volatile compound found in many plants, and its effects on insects can be either attractive or repellent, depending on the species.. However, the behavioral response of Orthaga achatina to vanillin, a volatile compound emitted by camphor trees, remains unexplored.

Article Title: Mechanism of circRNA_4083 Circularization and Its Role in Regulating Cell Viability.
Article Snippet: Primers were designed using Primer 5.0 (PREMIER Biosoft, San Francisco, CA, USA) and Oligo 7 (Molecular Biology Insights, San Francisco, CA, USA) with GAPDH as the endogenous control.

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae)
Article Snippet: Primer 5.0 ( http://www.premierbiosoft.com/ , accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Decoding the Olfactory Deception: Molecular Basis into Chilo suppressalis ' Recognition of the Lethal Trap Plant Vetiveria zizanioides .
Article Snippet: The striped stem borer (Chilo suppressalis), a devastating rice (Oryza sativa L.) pest, exhibits a paradoxical oviposition preference for vetiver grass (Vetiveria zizanioides L.), a nonhost plant causing complete larval mortality.. To elucidate the molecular basis of this maladaptive attraction, we investigated the olfactory mechanisms driving C. suppressalis’ recognition of vetiver volatiles.. Experimental results demonstrated that C. suppressalis exhibited a significant oviposition preference for vetiver over its natural host rice, despite the developmental incompatibility of offspring on the nonhost plant.

Article Title: Key Volatiles and ORs Mediating Oviposition Preference for Maize Plants in Chinese Population of Spodoptera frugiperda .
Article Snippet: Spodoptera frugiperda is a global pest that shows distinct preferences between maize and rice hosts.. Concerning the prevailing S. frugiperda corn strains in China, we identified five maize-specific volatiles through GC-MS analysis of volatiles from maize and rice seedlings, in which benzothiazole and nonadecane were confirmed as key volatiles mediating attractants for female moths.. Then, we screened for candidate odorant receptors (ORs) by analyzing differentially expressed genes between the moths exposed and unexposed to the active volatiles.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae).
Article Snippet: Primer 5.0 (http://www.premierbiosoft.com/, accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Molecular mechanisms based on peripheral level of vanillin recognition in Orthaga achatina (Lepidoptera: Pyralidae).
Article Snippet: Orthaga achatina (Lepidoptera Pyralidae) is a specialist pest of the camphor tree Cinnamomum camphora.. Vanillin is a volatile compound found in many plants, and its effects on insects can be either attractive or repellent, depending on the species.. However, the behavioral response of Orthaga achatina to vanillin, a volatile compound emitted by camphor trees, remains unexplored.

Article Title: Mechanism of circRNA_4083 Circularization and Its Role in Regulating Cell Viability.
Article Snippet: Primers were designed using Primer 5.0 (PREMIER Biosoft, San Francisco, CA, USA) and Oligo 7 (Molecular Biology Insights, San Francisco, CA, USA) with GAPDH as the endogenous control.

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae)
Article Snippet: Primer 5.0 ( http://www.premierbiosoft.com/ , accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Decoding the Olfactory Deception: Molecular Basis into Chilo suppressalis ' Recognition of the Lethal Trap Plant Vetiveria zizanioides .
Article Snippet: The striped stem borer (Chilo suppressalis), a devastating rice (Oryza sativa L.) pest, exhibits a paradoxical oviposition preference for vetiver grass (Vetiveria zizanioides L.), a nonhost plant causing complete larval mortality.. To elucidate the molecular basis of this maladaptive attraction, we investigated the olfactory mechanisms driving C. suppressalis’ recognition of vetiver volatiles.. Experimental results demonstrated that C. suppressalis exhibited a significant oviposition preference for vetiver over its natural host rice, despite the developmental incompatibility of offspring on the nonhost plant.

Article Title: Key Volatiles and ORs Mediating Oviposition Preference for Maize Plants in Chinese Population of Spodoptera frugiperda .
Article Snippet: Spodoptera frugiperda is a global pest that shows distinct preferences between maize and rice hosts.. Concerning the prevailing S. frugiperda corn strains in China, we identified five maize-specific volatiles through GC-MS analysis of volatiles from maize and rice seedlings, in which benzothiazole and nonadecane were confirmed as key volatiles mediating attractants for female moths.. Then, we screened for candidate odorant receptors (ORs) by analyzing differentially expressed genes between the moths exposed and unexposed to the active volatiles.

Quantitative RT-PCR:

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae).
Article Snippet: Primer 5.0 (http://www.premierbiosoft.com/, accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Molecular mechanisms based on peripheral level of vanillin recognition in Orthaga achatina (Lepidoptera: Pyralidae).
Article Snippet: Orthaga achatina (Lepidoptera Pyralidae) is a specialist pest of the camphor tree Cinnamomum camphora.. Vanillin is a volatile compound found in many plants, and its effects on insects can be either attractive or repellent, depending on the species.. However, the behavioral response of Orthaga achatina to vanillin, a volatile compound emitted by camphor trees, remains unexplored.

Article Title: Mechanism of circRNA_4083 Circularization and Its Role in Regulating Cell Viability.
Article Snippet: Primers were designed using Primer 5.0 (PREMIER Biosoft, San Francisco, CA, USA) and Oligo 7 (Molecular Biology Insights, San Francisco, CA, USA) with GAPDH as the endogenous control.

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae)
Article Snippet: Primer 5.0 ( http://www.premierbiosoft.com/ , accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Decoding the Olfactory Deception: Molecular Basis into Chilo suppressalis ' Recognition of the Lethal Trap Plant Vetiveria zizanioides .
Article Snippet: The striped stem borer (Chilo suppressalis), a devastating rice (Oryza sativa L.) pest, exhibits a paradoxical oviposition preference for vetiver grass (Vetiveria zizanioides L.), a nonhost plant causing complete larval mortality.. To elucidate the molecular basis of this maladaptive attraction, we investigated the olfactory mechanisms driving C. suppressalis’ recognition of vetiver volatiles.. Experimental results demonstrated that C. suppressalis exhibited a significant oviposition preference for vetiver over its natural host rice, despite the developmental incompatibility of offspring on the nonhost plant.

Article Title: Key Volatiles and ORs Mediating Oviposition Preference for Maize Plants in Chinese Population of Spodoptera frugiperda .
Article Snippet: Spodoptera frugiperda is a global pest that shows distinct preferences between maize and rice hosts.. Concerning the prevailing S. frugiperda corn strains in China, we identified five maize-specific volatiles through GC-MS analysis of volatiles from maize and rice seedlings, in which benzothiazole and nonadecane were confirmed as key volatiles mediating attractants for female moths.. Then, we screened for candidate odorant receptors (ORs) by analyzing differentially expressed genes between the moths exposed and unexposed to the active volatiles.

Generated:

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae).
Article Snippet: Primer 5.0 (http://www.premierbiosoft.com/, accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Molecular mechanisms based on peripheral level of vanillin recognition in Orthaga achatina (Lepidoptera: Pyralidae).
Article Snippet: Orthaga achatina (Lepidoptera Pyralidae) is a specialist pest of the camphor tree Cinnamomum camphora.. Vanillin is a volatile compound found in many plants, and its effects on insects can be either attractive or repellent, depending on the species.. However, the behavioral response of Orthaga achatina to vanillin, a volatile compound emitted by camphor trees, remains unexplored.

Article Title: Mechanism of circRNA_4083 Circularization and Its Role in Regulating Cell Viability.
Article Snippet: Primers were designed using Primer 5.0 (PREMIER Biosoft, San Francisco, CA, USA) and Oligo 7 (Molecular Biology Insights, San Francisco, CA, USA) with GAPDH as the endogenous control.

Article Title: Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of SWEET Genes Family in Four Cymbidium Species (Orchidaceae)
Article Snippet: Primer 5.0 ( http://www.premierbiosoft.com/ , accessed on 26 December 2022) was used to design primers for qRT-PCR analysis.

Article Title: Decoding the Olfactory Deception: Molecular Basis into Chilo suppressalis ' Recognition of the Lethal Trap Plant Vetiveria zizanioides .
Article Snippet: The striped stem borer (Chilo suppressalis), a devastating rice (Oryza sativa L.) pest, exhibits a paradoxical oviposition preference for vetiver grass (Vetiveria zizanioides L.), a nonhost plant causing complete larval mortality.. To elucidate the molecular basis of this maladaptive attraction, we investigated the olfactory mechanisms driving C. suppressalis’ recognition of vetiver volatiles.. Experimental results demonstrated that C. suppressalis exhibited a significant oviposition preference for vetiver over its natural host rice, despite the developmental incompatibility of offspring on the nonhost plant.

Article Title: Key Volatiles and ORs Mediating Oviposition Preference for Maize Plants in Chinese Population of Spodoptera frugiperda .
Article Snippet: Spodoptera frugiperda is a global pest that shows distinct preferences between maize and rice hosts.. Concerning the prevailing S. frugiperda corn strains in China, we identified five maize-specific volatiles through GC-MS analysis of volatiles from maize and rice seedlings, in which benzothiazole and nonadecane were confirmed as key volatiles mediating attractants for female moths.. Then, we screened for candidate odorant receptors (ORs) by analyzing differentially expressed genes between the moths exposed and unexposed to the active volatiles.



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